Inhibition of thymidine phosphorylation and DNA and histone synthesis in Ehrlich ascites carcinoma.
نویسندگان
چکیده
The time course of phosphorylation of thymidine and the effects of various inhibitors on the phosphorylation of thymidine and on DNA synthesis in Ehrlich ascites car cinoma cells in vitro were studied with a double-isotope labeling method. Hydroxyurea, mitomycin C, cycloheximide, puromycin, 1-0-D-arabinofuranosylcytosine, 5-fluoroorotic aldehyde, and the thiosemicarbazones of 2-phthalimido aliphatic aldehydes inhibit incorporation of thymidine-2-14C
منابع مشابه
Selective Inhibition by Sorbylhydroxamic Acid of Deoxyribonucleic Acid Synthesis in Ehrlich Ascites Tumor Cells1
Sorbylhydroxamic acid (SOHA) inhibited the incorporation in vitro of thymidine into deoxyribonucleic acid in Ehrlich ascites tumor cells, with no appreciable effect on the incorporation of uridine into ribonucleic acid or of L-leucine into protein. Hexanoylhydroxamic acid, devoid of the conjugated double-bond system, had no such activity. The concentration of SOHA conferring 50% inhibition of D...
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The aim of this study is to investigate the antitumor activity and possible molecular mechanism of Phenethyl isothiocyanate (PEITC) against Ehrlich ascites carcinoma in-vivo and in-vitro.In-vivo, ascetic fluid volume, body weight, serum malondialdehyde (MDA) level and total antioxidant capacity (TAC) were determined using Ehrlich ascites carcinoma (EAC) bearing mice. In-vitro, MTT assay was use...
متن کاملSelective inhibition by sorbylhydroxamic acid of deoxyribonucleic acid synthesis in Ehrlich ascites tumor cells.
Sorbylhydroxamic acid (SOHA) inhibited the incorporation in vitro of thymidine into deoxyribonucleic acid in Ehrlich ascites tumor cells, with no appreciable effect on the incorporation of uridine into ribonucleic acid or of L-leucine into protein. Hexanoylhydroxamic acid, devoid of the conjugated double-bond system, had no such activity. The concentration of SOHA conferring 50% inhibition of D...
متن کاملPhenethyl isothiocyanate Triggers Apoptosis, Combats Oxidative Stress and Inhibits Growth of Ehrlich Ascites Carcinoma Mouse Model
The aim of this study is to investigate the antitumor activity and possible molecular mechanism of Phenethyl isothiocyanate (PEITC) against Ehrlich ascites carcinoma in-vivo and in-vitro.In-vivo, ascetic fluid volume, body weight, serum malondialdehyde (MDA) level and total antioxidant capacity (TAC) were determined using Ehrlich ascites carcinoma (EAC) bearing mice. In-vitro, MTT assay was use...
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ورودعنوان ژورنال:
- Cancer research
دوره 30 11 شماره
صفحات -
تاریخ انتشار 1970